醫(yī)學(xué)免費(fèi)論文:加味桃核承氣湯對(duì)肝纖維化大鼠TGFβ1蛋白表達(dá)的影響

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醫(yī)學(xué)免費(fèi)論文:加味桃核承氣湯對(duì)肝纖維化大鼠TGFβ1蛋白表達(dá)的影響

【摘要】 [目的]觀察加味桃核承氣湯對(duì)四氯化碳肝纖維化大鼠肝組織轉(zhuǎn)化生長因子β1(TGFβ1)蛋白表達(dá)的影響。[方法]用四氯化碳誘導(dǎo)大鼠肝纖維化模型，研究分組為正常對(duì)照組、模型對(duì)照組、加味桃核承氣湯高、中、低劑量治療組和秋水仙堿對(duì)照組;灌胃給藥，療程8周;用免疫組化技術(shù)檢測(cè)肝組織TGFβ1的蛋白表達(dá);檢測(cè)血清ALT、AST水平;用苦味酸天狼紅膠原染色方法進(jìn)行半定量肝纖維化計(jì)分(SSS)。[結(jié)果]高、中、低劑量組及秋水仙堿組的TGFβ1的表達(dá)水平均低于模型對(duì)照組;經(jīng)加味桃核承氣湯治療后血清ALT、AST水平及肝纖維化SSS計(jì)分顯著下降。[結(jié)論]加味桃核承氣湯可抑制肝纖維化大鼠TGFβ1的表達(dá)，達(dá)到抗肝纖維化作用。

【關(guān)鍵詞】加味桃核承氣湯;四氯化碳;大鼠;肝纖維化

To Study the Effects of TGFβ1 of Jiaweitaohechengtang on Hepatic Fibrosis Induced by CCl4 in Rats Zhao Zhiyou1,Wu Yajun，Zhang Junjie2，et al No.117Hospital of PLA，Hangzhou(310013);2.Zhejiang University of TCM

Abstract：[Objective]To study the effects of TGFβ1 of Jiaweitaohechengtang on hepatic fibrosis induced by CCl4 in rats.[Methods]Hepatic fibrosis models were induced by CCl4 in rats.All the experimental rats were divided into six groups： normal control group，model control group，colchicine positive treatment group，highdose，mediumdose，lowdose jiaweitaohechenqitang treatment groups.Drugs were fed on rats for 8 weeks by intragastric administration.Protein expression levels of transfer growth factorsβ1(TGFβ1)were determined with immunohistochemical staining method.Scores of hepatic fibrosis were assessed with semiquantitive scoring system(SSS)through tianlanghong staining.[Results]The protein expression levels of TGFβ1 in the liver tissues in model control group were markedly increased compared with those in normal control group.Scores of hepatic fibrosis and the levels of serum ALT and AST in model control group were higher than those in normal control group，but lower than those in three treatment groups with jiaweitaohechengqitang.There were significant differences between model control group and three jiaweitaohechengqitang treatment groups.[Conclusion]Jiaweitaohechengqitang has therapeutic effects on hepatic fibrosis induced by CCl4 in rats醫(yī).學(xué)全.在.線網(wǎng)站gydjdsj.org.cn.

Key words：jiaweitaohechengqitang;CCl4;rat;hepatic fibrosis

肝纖維化是各種不同原因所致慢性肝病的共同病理特征，任其發(fā)展可致肝硬化，抗肝纖維化治療可阻斷其向肝硬化進(jìn)展。加味桃核承氣湯在臨床用于肝纖維化治療中取得了很好的療效，現(xiàn)對(duì)該方作深入研究，為臨床推廣應(yīng)用該方提供實(shí)驗(yàn)依據(jù)。

1 材料與方法

1.1 材料 (1)實(shí)驗(yàn)藥品：加味桃核承氣湯主要由桃仁、桂枝、大黃、甘草、莪術(shù)、白術(shù)、香附、茯苓等組成，用傳統(tǒng)方法浸泡、煎煮、過濾、濃縮，制成煎劑，每毫升含生藥2.7g，由浙江中醫(yī)學(xué)院門診部煎制;秋水仙堿：昆明制藥集團(tuán)股份有限公司。(2)實(shí)驗(yàn)動(dòng)物：SD大鼠，150只，體重160～200g，雌雄各半。由浙江中醫(yī)藥大學(xué)動(dòng)物實(shí)驗(yàn)中心提供。(3)主要試劑：四氯化碳(CCl4)分析純：上海凌峰化學(xué)試劑有限公司橄欖油：上海化學(xué)試劑公司;苦味酸、天狼紅：美國Sigma公司。兔抗鼠TGFβ1單克隆抗體：武漢博士德公司，二抗：美國Zymed公司;(4)主要儀器：切片機(jī)：德國Leca;生物顯微鏡：日本OLYMPUS CHK;高清晰度彩色病理圖文報(bào)告分析系統(tǒng)：武漢同濟(jì)醫(yī)科大學(xué);電子天平：上海第二天平衡器廠。