醫(yī)學(xué)免費論文:正糖鉗技術(shù)觀察降濁合劑對大鼠胰島素敏感性的影響

醫(yī)學(xué)免費論文:正糖鉗技術(shù)觀察降濁合劑對大鼠胰島素敏感性的影響

【摘要】 [目的]采用正糖鉗試驗觀察比較降濁合劑對Wistar大鼠胰島素敏感性的影響。[方法]雄性Wistar大鼠32只，隨機分成降濁合劑高劑量組、降濁合劑低劑量組、羅格列酮組、正常對照組各8只，各組灌予相應(yīng)的藥物7天后，大鼠腹腔麻醉，觀察大鼠正糖鉗試驗穩(wěn)態(tài)下的血糖(BG)和葡萄糖輸注速率(GIR)。[結(jié)果]降濁合劑可明顯降低大鼠正糖鉗試驗穩(wěn)態(tài)下的血糖(BG)，提高穩(wěn)態(tài)下的葡萄糖輸注速率(GIR)。[結(jié)論]降濁合劑能提高胰島素抵抗大鼠機體組織對外源性胰島素的敏感性。

【關(guān)鍵詞】 降濁合劑;羅格列酮;正糖鉗;胰島素敏感性

Fund project：Item of Zhejiang Provincial TCM Administration Bureau Foundation(No：2002C104)Observe the Effect of Jiangzhuo Mixture on Rats’ Insulin Sensitivity with Glucose Clamp Technology Gong Wenbo，Chen Xiabo，Zhou Jianyang，et al TCM Hospital of Ningbo City，Zhejiang Province(315010)

Abstract：[Objective] To observe the effect of jiangzhuo Mixture(JZM)on insulin sensitivity in Wistar rats by using the glucose clamp technique.[Methods]32 male Wistars were randomly divided into normal control group，control group treated with rosiglitazone and two experimental groups treated with high or low dose JZM.After durations of 7 days with corresponding treatment by intragastric administration，the blood glucose(BG)in homeostasis and glucose infusion rate(GIR)of each group is observed by using the glucose clamp technique.[Results]JZM can decrease the blood glucose(BG)in homeostasis and increase glucose infusion rate(GIR)obviously.[Couclusion]JZM can improve the insulin sensitivity in insulin�瞨esistance�瞨ats醫(yī).學(xué).全.在.線www.gydjdsj.org.cn.

Key words： jiangzhuo Mixture(JZM);rosiglitazone;glucose clamp technique;insulin sensitivity

中藥復(fù)方降濁合劑是根據(jù)我院國家級名老中醫(yī)、主任中醫(yī)師王暉多年臨床用藥經(jīng)驗，由我院制劑室配置的純中藥制劑，功能為補氣升清降濁，該藥在臨床應(yīng)用中對于治療2型糖尿病屬肥胖氣虛痰濁型有較好的療效[1]。本研究觀察了降濁合劑對胰島素鉗夾Wistar大鼠穩(wěn)態(tài)下的血糖(BG)和葡萄糖輸注速率(GIR)的影響，并與陽性對照藥—羅格列酮進(jìn)行了比較。

1 實驗材料

1.1 藥品 降濁合劑濃縮液：棕色液體、500ml/瓶，生藥濃度5g/ml，組成：生黃芪、丹參、生蒼術(shù)、生薏苡仁、生麥芽、生扁豆、絞股藍(lán)、生雞金、生葛根，寧波市中醫(yī)院提供。羅格列酮：商品名文迪雅，4mg/片，批號：H20020475。胰島素注射液：江蘇萬邦生化醫(yī)藥股份有限公司，400U/10ml/瓶，批號：0404208。

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