醫(yī)學論文范文:根除幽門螺桿菌對慢性胃炎和胃潰瘍組織中MIF蛋白表達的影響

醫(yī)學論文范文:根除幽門螺桿菌對慢性胃炎和胃潰瘍組織中MIF蛋白表達的影響

【摘要】 目的 通過檢測幽門螺桿菌(Hp)感染及根除治療對慢性胃炎和胃潰瘍組織中巨噬細胞移動抑制因子(MIF)蛋白表達的影響，探討MIF和Hp在慢性胃炎和胃潰瘍發(fā)生發(fā)展中的作用。方法 胃鏡下隨機采集胃黏膜活檢組織，經(jīng)14C呼氣試驗、Warthin�瞫tarry銀染色法檢測Hp均為陽性，并經(jīng)病理檢驗證實為淺表性胃炎、萎縮性胃炎、胃潰瘍各40例，Hp陰性的健康檢查者25例，采用免疫組織化學SP方法分別檢測各組MIF蛋白的表達;進行Hp根治治療2周，停藥4周后復查Hp及MIF，比較根除前后各組MIF水平。結果 MIF蛋白在Hp陰性的正常胃黏膜中陽性表達低(2/25，8%);在Hp感染的慢性淺表性胃炎(12/40，30%)、萎縮性胃炎(26/40，65%)和胃潰瘍組織(19/40，47.5%)陽性表達增強，顯著高于Hp陰性的正常胃黏膜組(57/120 vs. 2/25;χ2=13.376，P<0.01);在慢性炎癥中，隨著炎癥程度增加，MIF表達增強，差異有顯著性(12/40 vs. 26/40;χ2=9.825，P<0.01);Hp根除后，MIF蛋白表達陽性率較治療前顯著降低(57/120 vs. 23/103;χ2=15.264, P<0.01)，而Hp仍為陽性者MIF蛋白陽性率無顯著變化。結論 Hp感染導致的胃黏膜慢性炎癥及潰瘍的形成與胃黏膜MIF的表達相關，根除Hp感染可降低胃黏膜MIF基因表達的異常，可能對預防或減緩胃癌的發(fā)生和發(fā)展具有重要意義。

【關鍵詞】 巨噬細胞移動抑制因子;幽門螺桿菌;慢性胃炎;胃潰瘍

Effect of Helicobacter pylori infection on macrophage migration　inhibitory factor protein expression in patients withchronic gastritis and gastric ulcer precancerous lesions

ZHANG Shuang�瞲ia, LI Guang�瞕i, YU Xiao�瞙ui, ZHANG Fang�瞲in

1. the Second Clinical Medical College of Lanzhou University, Lanzhou 730000;2. Department of Laboratory, the Second Affiliated Hospital of　Lanzhou University, Lanzhou 730000; 3. Department of Gastroenterology,Lanzhou General Hospital of Lanzhou Command, Lanzhou 730000, China醫(yī).學全.在.線gydjdsj.org.cn

ABSTRACT: Objective To investigate the effect of Helicobacter pylori (Hp) infection on macrophage migration inhibitory factor (MIF) protein expression and explore the role of Hp and MIF in the development of chronic gastritis and gastric ulcer. Methods The biopsy tissues of gastric mucosa were collected under gastroscope, and Hp was detected by 14C breath test and Warthin�瞫tarry method. We recruited 25 healthy people with normal gastric mucosa, 40 patients pathologically confirmed Hp�瞤ositive with chronic superficial gastritis, 40 with atrophic gastritis and 40 with gastric ulcer. MIF protein expression was examined by immunohistochemical SP staining method, then Hp eradication was performed on Hp�瞚nfected chronic superficial gastritis, atrophic gastritis and gastric ulcer for 2 weeks. Hp and MIF were re�瞖xamined 4 weeks after drug withdrawal, and difference in MIF expression was compared between Hp�瞚nfected patients and Hp�瞖radicated patients. Results The expression of MIF was low in normal gastric mucosa without Hp infection (2/25, 8%), but significantly higher in Hp�瞚nfected gastric mucosa with chronic superficial gastritis (12/40, 30%), atrophic gastritis (26/40, 65%) and gastric ulcer (19/40, 47.5%); there was a significant difference between normal gastric mucosa without Hp infection and that of　Hp�瞚nfected patients (57/120 vs. 2/25; χ2=13.376, P<0.01). MIF expression increased with the severity of inflammation in chronic gastritis, and there was a significant difference between superficial gastritis and atrophic gastritis (12/40 vs. 26/40; χ2=9.825, P<0.01). The expression of MIF was noticeably decreased after Hp eradication compared with before(57/120 vs. 23/103; χ2=15.264, P<0.01); however, there was no significant change in those patients whose Hp was still positive. Conclusion The expression of MIF on gastric mucosa is associated with the development of chronic gastritis and gastritis ulcer caused by Hp infection. Eradication of Hp could cut down the abnormally high MIF expression in gastric mucosa and slow down the formation and development of gastric carcinoma.

KEY WORDS: macrophage migration inhibitory factor; Helicobacter pylori; chronic gastritis; gastric ulcer

巨噬細胞移動抑制因子(macrophage migration inhibitory factor, MIF)是重要的炎癥因子，能抑制巨噬細胞遷移，促進IV型過敏反應中巨噬細胞的聚積。近年來，許多研究證明MIF參與細胞增殖和分化的調節(jié)，在多種炎癥性疾病和惡性腫瘤細胞中高表達，在胃癌中的表達顯著增高[1��2]，幽門螺桿菌(helicobacter pylori, Hp)是慢性胃炎、消化性潰瘍、胃癌和胃B細胞淋巴瘤重要的危險因子[3]。為進一步探討Hp和MIF在慢性胃炎胃潰瘍向胃癌發(fā)生過程中的作用，我們檢測了Hp感染的慢性淺表性胃炎、萎縮性胃炎和胃潰瘍組織中MIF的表達，并給予Hp根除治療，分析Hp根除前后MIF表達的變化，探討MIF和Hp在慢性胃炎和胃潰瘍發(fā)生發(fā)展過程中的可能作用及兩者的相關性。

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