醫(yī)學(xué)論文范文:熱休克蛋白70��1基因多態(tài)性與冠心病的關(guān)聯(lián)性研究

醫(yī)學(xué)論文范文:熱休克蛋白70��1基因多態(tài)性與冠心病的關(guān)聯(lián)性研究

【摘要】 目的：探討熱休克蛋白70��1(HSP70��1)基因多態(tài)性與冠心病的關(guān)系。方法：采用病例-對(duì)照1:1研究設(shè)計(jì)。選擇湖北省三家綜合性醫(yī)院的217例冠心病患者作為病例組，同時(shí)選年齡、性別匹配的217例健康者作為對(duì)照組。采用聚合酶鏈反應(yīng)-限制性片斷長度多態(tài)性(PCR�睷FLP)方法檢測HSP70��1多態(tài)性。結(jié)果：HSP70��1基因各基因型及等位基因頻率在兩組中的分布差異有統(tǒng)計(jì)學(xué)意義。結(jié)論：HSP70��1基因多態(tài)性可能是冠心病的易感基因。

【關(guān)鍵詞】 熱休克蛋白70 基因多態(tài)性 冠心病

Association Study of Heat Shock Protein 70��1 Gene Polymorphism and Coronary Heart Disease LIU Yong1,2,YANG Han�瞕ong2^,MIN Xin�瞱en2,LI Dong�瞗eng2 (1Department of Cardiovascular Disease,Renmin Hospital,Wuhan University,Wuhan Hubei 430061;2Department of Cardiovascular Disease,Dongfeng Hospital,Yunyang Medical College, Shiyan, Hubei 442000, China)

Abstract：Objective To explore the association between gene polymorphism of heat shock protein 70��1(HSP70��1) and the susceptibility to coronary heart disease. Methods A matched case�瞔ontrol study was performed.Two hundreds and seventeen patients with coronary heart disease were selected as case group from three general hospitals in Hubei province,and 217 healthy subjects with age and gender matched were simultaneously selected from the same geographic area as control. HSP70��1 genotypes were determined with polymerase chain reaction�瞨estriction fragment length polymorphism(PCR�睷FLP).Results There were significant diferences in the distribution of HSP70��1 genotypes and allele frequency between case group and control group.Conclusion HSP70��1 gene polymorphism may be one of genetic susceptibility factors of coronary heart disease.

Key words：Heat shock protein 70;Gene polymorphism;Coronary heart disease

冠心病(coronary heart disease，CHD)是一種常見于40歲以上中老年人的多病因疾病，也是危害職業(yè)人群健康最常見的疾病之一。隨著冠心病發(fā)病率及死亡率的上升，對(duì)其病因?qū)W方面的研究已成為目前研究的重點(diǎn)和熱點(diǎn)，但其發(fā)病機(jī)制尚不清楚。熱休克蛋白(heat shock protein，HSP)又稱為應(yīng)激蛋白，是生物細(xì)胞在受熱、生物應(yīng)激、理化因素應(yīng)激原刺激后，所產(chǎn)生的一類在生物進(jìn)化中最保守并由熱休克基因所編碼的伴隨細(xì)胞蛋白。在許多情況下HSP70在心血管系統(tǒng)優(yōu)先表達(dá)，尤其是在血量豐富的組織，如腦、肝臟、肺臟、心臟等，這說明HSP70與心血管系統(tǒng)有密切的聯(lián)系。為研究冠心病患者與HSP70��1基因多態(tài)性的相關(guān)性本課題進(jìn)行了初步探索醫(yī).學(xué).全.在.線www.gydjdsj.org.cn。

1 對(duì)象與方法

1.1 研究對(duì)象

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