醫(yī)學(xué)論文范文:壓力超負(fù)荷大鼠腎組織脂質(zhì)過氧化的變化及替米沙坦干預(yù)作用

醫(yī)學(xué)論文范文:壓力超負(fù)荷大鼠腎組織脂質(zhì)過氧化的變化及替米沙坦干預(yù)作用

【摘要】目的：探討腹主動脈縮窄法模擬壓力超負(fù)荷大鼠模型腎組織脂質(zhì)過氧化、腎功能改變及替米沙坦干預(yù)作用. 方法：將36只Wistar大鼠隨機(jī)等分成3組: 假手術(shù)組(SG)，腹主動脈縮窄組(OG)，替米沙坦+腹主動脈縮窄組(TM＋OG),每組12只. 采用腹主動脈縮窄法制備壓力超負(fù)荷大鼠模型，分別測定大鼠血清肌酐(Cr)和尿素氮(BUN)含量及大鼠腎組織勻漿丙二醛(MDA)、谷胱甘肽(GSH)含量. 結(jié)果：腹主動脈縮窄組(OG)及替米沙坦+腹主動脈縮窄組(TM＋OG)血清Cr, BUN含量及腎組織勻漿MDA水平均較假手術(shù)組(SG)增高，但TM＋OG低于OG；腎組織勻漿GSH水平OG及TM＋OG低于SG，但TM＋OG高于OG. 結(jié)論：腹主動脈縮窄法制備壓力超負(fù)荷大鼠腎臟脂質(zhì)過氧化物改變明顯且替米沙坦有保護(hù)作用.

【關(guān)鍵詞】  腎臟; 壓力超負(fù)荷; 脂質(zhì)過氧化; 替米沙坦醫(yī).學(xué)全.在.線網(wǎng)站gydjdsj.org.cn

 Change of lipid peroxidation of nephric tissue in pressure�瞣verloaded rat model as well as intervention of telmisartan

ZHOU Jian�睪uang1, LIU Zhao�睠huan2, MU Xian�瞃ou2, WANG Xu�睰ai3

1Department of Clinical Laboratory, 2Department of Cardiology, Chinese PLA 252 Hospital,  Baoding 071000, China,3Depart�瞞ent of Cardiology, Daping Hospital, Third Military Medical University, Chongqing 400042, China

【Abstract】 AIM: To illustrate the change in lipid peroxidation of pressure�瞣verloaded rat model produced by abdominal aorta constriction as well as the intervention effects of telmisartan. METHODS: Thirty�瞫ix Wistar rats were divided into 3 groups: sham�瞣peration group (SG), abdominal aorta constriction group (operation group, OG), and telmisartan + operation group (TM+OG), with 12 in each group. Pressure�瞣verloaded rat models  were established by using abdominal aorta constriction method. The levels of creatinine (Cr) and urea nitrogen (BUN) in the rats�� serum as well as those of malonaldehyde (MDA) and glutathion (GSH) of the rats�� nephric tissue were measured respectively. RESULTS: Compared with SG, the levels of Cr and BUN and that of MDA in the nephric tissue were significantly higher than those in OG and TM+OG. The levels of these indices in TM+OG were lower than that in OG. The level of GSH in OG and TM+OG was lower than that in SG, but higher in TM+OG than in OG. CONCLUSION: The change of the lipid peroxidation in the pressure�瞣verloaded rat model is obvious and telmisartan exerts the protective effects against the changes醫(yī).學(xué)全.在.線網(wǎng)站gydjdsj.org.cn.

【Keywords】 nephridial tissue; pressure overloads; lipid peroxidation; telmisartan

0引言

心、腎是壓力超負(fù)荷重要的靶器官，長期壓力超負(fù)荷可導(dǎo)致心肌細(xì)胞肥大，并最終發(fā)生慢性心功能不全，心功能不全時腎功能惡化已成為臨床工作中的重要問題. 替米沙坦是新一代長效、高效、低毒的新型血管緊張素Ⅱ受體阻滯藥. 本文通過腹主動脈縮窄法模擬壓力超負(fù)荷大鼠模型探討腎臟脂質(zhì)過氧化物變化以及血管緊張素Ⅱ受體I型拮抗劑替米沙坦對壓力超負(fù)荷大鼠腎臟損壞的保護(hù)作用.

1材料和方法

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