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醫(yī)學(xué)論文范文:甘珀酸對(duì)鐵離子誘導(dǎo)的癲癇大鼠縫隙連接及腦電的影響
【摘要】 目的：探究縫隙連接在外傷性癲癇(posttraumatic epilepsy，PTE)發(fā)病機(jī)制中的作用。方法：大鼠隨機(jī)分為正常組、假手術(shù)組、模型組和甘珀酸干預(yù)組。觀察大鼠行為學(xué)表現(xiàn)，并作腦電圖動(dòng)態(tài)記錄。結(jié)果：大鼠行為學(xué)上出現(xiàn)癲癇發(fā)作，腦電圖描記出多種形式的癲癇樣放電波形。與模型組相比，應(yīng)用甘珀酸干預(yù)后，大鼠行為學(xué)發(fā)作明顯減少，程度減輕，癇性放電潛伏期不同程度延長(zhǎng)，頻率明顯減慢和波幅明顯降低，差別具有統(tǒng)計(jì)學(xué)意義(P<0.05)。結(jié)論：腹腔注射甘珀酸可明顯減輕癇性發(fā)作，減少癇性放電頻率，降低波幅，提示大量異常的縫隙連接生成可能在PTE形成中起到了重要作用。
【關(guān)鍵詞】 外傷性癲癇;鐵離子;縫隙連接;甘珀酸;腦電圖
The intervention of carbenoxolone to gap junction and the EEG of Fe3+ induced epilepsy in rat
CHEN Weiguan1，GAO Zhiwei2，JIANG Zhenglin3，et al (1Department of Rehabilitation Medicine, the Affiliated Hospital of Nantong University，2Department of Neurology,3Institute of Nautical Medicine，Nantong University，Nantong 226001)
[Abstract] Objective: To explore the role of gap junction in the posttraumatic epilepsy. Methods: Rats were divided into four groups: normal, sham operated, model and carbenoxolone intervention. Behavior of epilepsy was observed and EEG was recorded. Results: Rats displayed typical epileptic behaviors, and epileptic discharges were observed at the same time. Carbenoxolone reduced seizure degree, prolonged the latencies of epileptic discharge, and decreased frequency and amplitude of epileptic waves (P<0.05). Conclusions: Intraperitoneal administration of carbenoxolone could reduce seizure degree. Gap junction might play a role in posttraumatic epilepsy.
[Key words] Posttraumatic epilepsy; FeCl3; Gap junction; carbenoxolone; Electroencephalogram
外傷性癲癇(posttraumatic epilepsy，PTE)是一種繼發(fā)于腦部創(chuàng)傷后，反復(fù)的癇性發(fā)作性疾病，可能出現(xiàn)在頭部外傷后的數(shù)年[1]。它在原發(fā)損傷的基礎(chǔ)上進(jìn)一步加重了腦組織的病理?yè)p傷及神經(jīng)生化改變，惡化病情，增大死亡風(fēng)險(xiǎn)，使臨床處置更趨復(fù)雜，給患者帶來了極大的精神負(fù)擔(dān)和就業(yè)壓力，同時(shí)也給家庭和社會(huì)帶來了沉重的經(jīng)濟(jì)負(fù)擔(dān)，因此這是一個(gè)巨大的醫(yī)學(xué)和社會(huì)問題。目前對(duì)PTE缺乏有效的防治方法，絕大部分患者可發(fā)展為難治性癲癇，其主要原因是對(duì)PTE的發(fā)病機(jī)制尚未清楚。我們通過建立鐵誘導(dǎo)的大鼠PTE模型，探討縫隙連接(gap junction，GJ)阻斷劑甘珀酸在PTE防治中的應(yīng)用及相關(guān)機(jī)制，為GJ阻斷劑用于臨床治療PTE提供必要的信息醫(yī).學(xué).全.在.線gydjdsj.org.cn。
1 材料與方法
