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醫(yī)學(xué)論文范文:粉防己堿誘導(dǎo)宮頸癌細(xì)胞凋亡的定性定量研究
【摘要】 目的 從定性定量角度探討粉防己堿誘導(dǎo)宮頸癌HeLa細(xì)胞凋亡的作用。方法 采用MTT法檢測不同濃度粉防己堿作用不同時(shí)間對(duì)人宮頸癌HeLa細(xì)胞株的增殖抑制作用。通過流式細(xì)胞儀和激光共聚焦顯微鏡檢測細(xì)胞凋亡情況。結(jié)果 粉防己堿對(duì)宮頸癌HeLa細(xì)胞具有增殖抑制作用，且呈時(shí)間、濃度依賴性。應(yīng)用流式細(xì)胞儀檢測到15μmol/L粉防己堿誘導(dǎo)HeLa細(xì)胞產(chǎn)生的凋亡率為(51.8±0.97)%，顯著高于對(duì)照組凋亡率(24.3±1.23)%(P<0.05)。通過激光共聚焦顯微鏡觀察，與對(duì)照組比較，粉防己堿作用后HeLa細(xì)胞具有明顯的凋亡形態(tài)。結(jié)論 粉防己堿能夠抑制HeLa細(xì)胞的增殖并誘導(dǎo)其凋亡。
【關(guān)鍵詞】 粉防己堿;宮頸癌;凋亡;
Qualitative and quantitative study on the apoptosis of cervical cancer cells treated by tetrandrineZHU Ke-xiu, LI Bin, DENG Zhuo, YUAN Cai-ping, Mu Jian-ning, HAN Xiao-bingDepartment of Obstetrics & Gynecology, the First Affiliated Hospital,Medical School of Xian Jiaotong University, Xian 710061, ChinaABSTRACT: Objective To study the effects of tetrandrine on apoptosis of HeLa cervical cancer cells qualitatively and quantitatively. Methods We measured tetrandrine-induced inhibition of HeLa cell proliferation at different concentrations and time points by MTT assay. The rate of Hela cell apoptosis induced by tetrandrine was detected by flow cytometer and confocal laser scanning microscope (CLSM). Results Tetrandrine inhibited the proliferation of HeLa cells in dosage- and time-dependent manners. Flow cytometry showed that the apoptosis rate was (51.8±0.97)% at the concentration of 15μmol/L, which was significantly higher than that in the control group (24.3±1.23)% (P<0.05). The cells treated with tetrandrine showed typical apoptotic morphology under CLSM. Conclusion Tetrandrine can inhibit proliferation and induce apoptosis of HeLa cervical cancer cells.
KEY WORDS: tetrandrine; cervical cancer; apoptosis醫(yī).學(xué).全.在.線gydjdsj.org.cn
全球每年估計(jì)約有470000例新增的宮頸癌病例，并且每年約有230000例宮頸癌患者死亡。發(fā)展中國家占80%，在發(fā)展中國家的很多地區(qū)，宮頸癌是最常見的腫瘤[1-2]。早期宮頸癌的治療主要是通過手術(shù)，進(jìn)展期的宮頸癌主要通過體外放射線和體腔內(nèi)放射線治療。近年來有很多關(guān)于放、化療結(jié)合的研究。但是，放療和化療以及放、化療均有明顯的副作用，所以能否利用一些毒副作用較小的藥物來治療宮頸癌是一個(gè)值得研究的問題。
粉防己堿(tetrandrine, Tet)是從中草藥防己的根塊中提取的雙芐基異喹啉類生物堿，具有廣泛的藥理作用，臨床用于治療關(guān)節(jié)炎、高血壓、心率失常和肺纖維化等疾病具有較好的療效[3-8]。許多研究認(rèn)為粉防己堿是一種有前途的抗癌藥物，可以抑制多種腫瘤細(xì)胞的生長[9-14]。然而，粉防己堿對(duì)宮頸癌細(xì)胞是否具有抑制增殖的作用尚未見報(bào)道。本實(shí)驗(yàn)以宮頸癌HeLa細(xì)胞為處理對(duì)象，以不同劑量的粉防己堿處理，觀察其誘導(dǎo)HeLa細(xì)胞凋亡的作用，為抗宮頸癌藥物治療提供新的思路。
