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醫(yī)學(xué)免費(fèi)論文:雙歧桿菌在兒童口腔的分布及與兒童齲病的關(guān)系
【摘要】 目的 探索一種可以較好地分離口腔雙歧桿菌的選擇性培養(yǎng)基，以此為基礎(chǔ)，檢測兒童口腔不同部位雙歧桿菌的分布狀態(tài)，初步探討口腔雙歧桿菌與兒童齲病的發(fā)生關(guān)系。方法 選擇70例3~6歲兒童為研究對象，其中無齲組30例，重癥嬰幼兒齲(S-ECC)組40例。無齲組收集唾液及唇面、鄰面、面溝裂的混合菌斑;S-ECC組收集唾液、光滑面菌斑、牙面白堊斑菌斑和深齲齲壞組織。在經(jīng)過改良的TPY選擇性培養(yǎng)基中進(jìn)行培養(yǎng)，提取細(xì)菌總DNA，用雙歧桿菌特異性引物進(jìn)行聚合酶鏈反應(yīng)，對其電泳條帶進(jìn)行記錄分析。結(jié)果 S-ECC組雙歧桿菌的檢出率為47.5%，無齲組為0，2組間檢出率的差異有統(tǒng)計(jì)學(xué)意義(P<0.05)。在S-ECC組兒童口腔的不同部位，雙歧桿菌檢出率分別為唾液27.5%、光滑面菌斑27.5%、白堊斑菌斑20.0%、齲壞組織22.5%，不同檢測部位間檢出率的差異無統(tǒng)計(jì)學(xué)意義(P>0.05)。齒雙歧桿菌在S-ECC組兒童口腔不同部位的檢出率分別為唾液10.0%、光滑面菌斑7.5%、白堊斑菌斑7.5%、齲壞組織10.0%，不同檢測部位間檢出率的差異也無統(tǒng)計(jì)學(xué)意義(P>0.05)。結(jié)論 改良TPY雙歧桿菌選擇性培養(yǎng)基是一種適合分離口腔雙歧桿菌的選擇性培養(yǎng)基;雙歧桿菌可能與S-ECC的發(fā)生相關(guān)，但其檢出情況與兒童牙面的部位關(guān)系不大。
【關(guān)鍵詞】 重癥嬰幼兒齲; 雙歧桿菌; 齒雙歧桿菌; 聚合酶鏈反應(yīng)
Distribution of Bifidobacterium in oral cavities of children and the relations with caries ZHAI Jing-jing1, ZOU Jing2, LU Li-ying3. (1. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China; 2. Dept. of Pediatric Dentistry, West China College of Stomatology, Chengdu 610041, China; 3. Dept. of Pediatric Dentistry, Chinese PLA General Hospital, Beijing 100853, China醫(yī).學(xué).全.在.線gydjdsj.org.cn)
[Abstract] Objective To explore a selected-media of Bifidobacterium from oral cavity, to detect the distribution of Bifidobacterium in different sites of children and primarily investigate the relationship between oral Bifidobacterium and early childhood caries. Methods 70 children aged from 3 to 5-year-old were selected, 30 children were caries-free and 40 were severe early childhood caries(S-ECC). Saliva was collected and plaque samples from the 30 healthy subjects were pooled. For S-ECC group, plaques were collected separately from four different sites as follows: Saliva, surfaces of intact enamel, surfaces of white spot-lesions, and deep dentin-lesions. Samples would be grown in the selected-media, and the whole DNA of bacteria was extracted. Polymerase chain reaction was performed with specific primers and the results were analyzed by the electrophoresis. Results Bifidobacterium were detected 0 in the caries-free children, while 47.5% in the S-ECC group. There was significant difference between two groups(P<0.05) and there was no difference between different sites of teeth in S-ECC group(P>0.05). 27.5% Bifidobacteriumwere detected in saliva, 27.5% on surfaces of intact enamel, 20.0% on surfaces of white spot-lesions and 22.5% in deep dentin-lesions. 10% Bifidobacterium dentium were detected in saliva, 7.5% on surfaces of intact enamel, 7.5%on surfaces of white spot-lesions and 10.0% in deep dentin-lesions. Conclusion One type of modified selected media of Bifidobacterium in oral cavity was explored. Bifidobacterium may be related to the occurrence of the S-ECC andhas nothing to do with different sites of teeth in children.
[Key words] severe early childhood caries; Bifidobacterium; Bifidobacterium dentium; polymerase chain reaction
