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醫(yī)學(xué)免費(fèi)論文:細(xì)胞調(diào)控基因hCHK2多態(tài)性與食管癌易感性的研究
【關(guān)鍵詞】 細(xì)胞調(diào)控基因 hCHK2多態(tài)性 食管癌易感性
susceptibility of esophageal cancer ZHANG Huixia, CHEN Yan, YIN Dong, et al
(School of Public Health, Xinjiang Medical University, Urumqi 830011, China)Abstract: Objective: To determine the association between hCHK2 polymorphism and the susceptibility of esophageal cancer in Han nationality. Methods: A casecontrol study was conducted. The polymorphism between hCHK2 gene at rs2278022、rs2602431 and rs2970077 position was analyzed by polymerase chain reactionligase detection reaction (PCRLDR) methods in 107 esophageal cancer cases and 204 noncancer controls. The frequencies of genotype and allele and the association between the genetic polymorphisms and cancer risk were analyzed. Results: No significant difference was observed in the frequency of hCHK2 gene at rs2278022、rs2602431 and rs2970077 position genotypes between cases and controls (P>0.05). Conclusion: The polymorphism of hCHK2 gene at rs2278022、rs2602431 and rs2970077 position is unlikely to be associated with the susceptibility of esophageal cancer醫(yī).學(xué).全.在.線gydjdsj.org.cn.
Key words: hCHK2; esophageal cancer; susceptibility; genetic polymorphism隨著對細(xì)胞周期及其調(diào)控的深入認(rèn)識，學(xué)者們提出了“癌癥可能是一類細(xì)胞周期異常性疾病”的觀點(diǎn)[1]。絕大多數(shù)腫瘤細(xì)胞都存在若干細(xì)胞周期調(diào)控機(jī)制的改變，周期調(diào)控機(jī)制的異常可導(dǎo)致細(xì)胞的持續(xù)性增殖，是腫瘤發(fā)生的生物學(xué)基礎(chǔ)。CHK2是近來新發(fā)現(xiàn)的一個(gè)細(xì)胞周期調(diào)控蛋白。在發(fā)生DNA損傷或復(fù)制阻滯后，細(xì)胞通過不同途徑激活CHK2，進(jìn)而作用于下游不同的靶蛋白，最終激活G1、S和(或)G2/M期檢測點(diǎn)機(jī)制，使細(xì)胞周期進(jìn)程發(fā)生阻滯，同時(shí)激活修復(fù)相關(guān)基因的轉(zhuǎn)錄，促進(jìn)細(xì)胞對損傷進(jìn)行修復(fù)。由于CHK2在細(xì)胞周期中具有重要的負(fù)調(diào)控作用，許多學(xué)者認(rèn)為CHK2起著抑癌基因的作用，它的異常會(huì)導(dǎo)致細(xì)胞周期檢測點(diǎn)對DNA損傷或復(fù)制阻滯不能發(fā)揮正常功能，從而引起腫瘤易感性增加，最終導(dǎo)致腫瘤的發(fā)生或發(fā)展。因此，我們采用聚合酶鏈?zhǔn)椒磻?yīng)連接酶檢測反應(yīng)(polymerase chain reactionligase detection reaction，PCRLDR)技術(shù)對新疆漢族人群107例食管癌患者和204例非食管癌患者進(jìn)行了hCHK2 rs2278022、rs2602431和rs2970077基因多態(tài)性的檢測，并分析探討了其基因多態(tài)性與食管癌遺傳易感性之間的關(guān)系，以期為食管癌的防治提供理論依據(jù)。
1材料與方法
1.1研究對象收集2005～2007年間新發(fā)的、由5所醫(yī)院(新疆醫(yī)科大學(xué)第一附屬醫(yī)院、第二附屬醫(yī)院、附屬腫瘤醫(yī)院、伊犁州新華醫(yī)院、伊犁州友誼醫(yī)院)經(jīng)內(nèi)窺鏡、X線及病理確診的漢族原發(fā)性食管癌患者107例作為病例組，其中男性76例，女性31例，并選擇與病例無血緣關(guān)系、同民族、同性別、年齡相差±5歲、同期住院的非食管癌患者204例作為對照組，其中男性151例，女性53例。所有研究對象采集外周靜脈血4 ml，EDTA抗凝，20℃保存至提取DNA。全部調(diào)查對象均知情同意。
1.2方法
