 |
 |
醫(yī)學(xué)論文范文:17β_雌二醇對新生牛血清誘導(dǎo)的血管平滑肌細胞增殖反應(yīng)的影響及其機制
【摘要】 目的：探討雌甾_1,3,5(10)_三烯_3，17β_二醇(17β_雌二醇)對新生牛血清誘導(dǎo)血管平滑肌細胞(VSMCs)增殖反應(yīng)的影響及其機制。方法：用新生牛血清刺激培養(yǎng)的VSMCs增殖，MTT法檢測17β_雌二醇對新生牛血清誘導(dǎo)的VSMCs增殖的影響，并用Western blot檢測caveolin_1蛋白，誘導(dǎo)型一氧化氮合酶(iNOS)及NO在此過程中的變化。結(jié)果：與對照組比，新生牛血清可促進VSMCs增殖(P<0.05)，17β_雌二醇作用24h后能明顯抑制上述作用(P<0.05);17β_雌二醇預(yù)處理可抑制新生牛血清誘導(dǎo)的VSMCs中caveolin_1，iNOS蛋白表達下降及NO釋放(P<0.05);17β_雌二醇受體阻斷劑1_{4_[2_(n,n_二甲氨基)乙氧基]苯｝_1,2_二苯_1_丁烯(他莫昔芬)預(yù)處理可部分逆轉(zhuǎn)17β_雌二醇的抑制作用(P<0.05)。結(jié)論：17β_雌二醇通過其受體可抑制新生牛血清誘導(dǎo)的VSMCs增殖，此過程與上調(diào)caveolin_1蛋白表達，激活iNOS，增加NO釋放有關(guān)。
【關(guān)鍵詞】 17β_雌二醇;caveolin_1;誘導(dǎo)型一氧化氮合酶;血管平滑肌細胞
Influence of 17β_estradiol on Vascular Smooth Muscle Cells Proliferation Induced
by Fetal Calf Serum and Its MechanismLIU Hai_mei1，ZHAO Xiao_feng2，XU Jin_wen3，LIN Gui_ping3，WANG Ting_huai3
(1 Department of Physiology，Guangzhou University of Chinese Medicine，Guangzhou 510006，China，2 Department of Physiology，Me_dical College of Shaoguan University，Shaoguan 512026，China，3 Department of Physiology，Zhongshan Medical College，Sun_Yat Sen University，Guangzhou 510080，China)
[Abstract]Objective： To investigate the influence of 17β_estradiol on vascular smooth muscle cells(VSMCs)proliferation induced by fetal calf serum and the related mechanism. Methods： The fetal calf serum was used to stimulate cultured VSMCs proliferation(P<0.05)，and the effects of estrogen on VSMCs proliferation were determined by MTT，and the expressions of caveolin_1 and inducible nitric oxide synthase(iNOS)were also detected by Western blot. Results： Compared with the normal control group， the fetal calf serum could increase the VSMCs proliferation(P<0.05)， and inhibit proliferation effect of fetal calf serum，pretreated with estradiol for 24 hours(P<0.05). Western bolt results further proved that 17β_estradiol inhibited the downregulation of caveolin_1， iNOS and NO induced by fetal calf serum， which was prevented by pretreatment with estrogen antagonist， Tamoxifen(P<0.05). Conclusion： 17β_estradiol can inhibit VSMCs proliferation induced by fetal calf serum， and this effect is mediated by estrogen receptor. 17β_estradiol increases caveolin_1 and iNOS protein expression， and NO release，leading to the inhibition of VSMCs proliferation induced by fetal calf serum醫(yī).學(xué)全.在.線網(wǎng)站gydjdsj.org.cn.
[Key Words]17β_estradiol，caveolin_1，inducible nitric oxide synthase，vascular smooth muscle cell
血管損傷后，刺激因子誘導(dǎo)的血管平滑肌細胞(VSMCs)的過度增殖和遷移是再狹窄發(fā)生的關(guān)鍵[1_2]，臨床及流行病學(xué)資料顯示，雌甾_1,3,5(10)_三烯_3，17β_二醇(17β_雌二醇)具有心血管保護作用，我室前期的實驗研究也證實這點[3]，在離體培養(yǎng)的VSMCs中17β_雌二醇可抑制ET_1誘導(dǎo)的VSMCs增殖，此過程與抑制細胞外信號通路1/2(ERK1/2)有關(guān)。17β_雌二醇的血管保護作用與誘導(dǎo)NO生成有關(guān)，但在VSMCs中，17β_雌二醇通過何種途徑誘導(dǎo)NO合成，目前尚未明了。因此，本研究利用離體培養(yǎng)的兔胸主動脈VSMCs，觀察caveolin_1及誘導(dǎo)型一氧化氮合酶(iNOS)在17β_雌二醇誘導(dǎo)NO合成及抑制VSMCs增殖中的作用和機制。
