引用本文：劉向輝;戈峰,.蚯蚓纖溶酶活性影響因子的研究[J].中國(guó)中藥雜志,2002,27(06):423.
中文摘要:目的 :對(duì)提取過程中影響蚯蚓纖溶酶活性的 5種因子進(jìn)行了分析 ,以提高纖溶酶的提取活性。方法 :分別采用 0.9%NaCl抽提、10%蔗糖抽提、75%酒精抽提出粗酶。酶活測(cè)定方法采用尿激酶瓊脂糖 纖維蛋白平板法。硒含量的測(cè)定方法采用 3,3′-二氨基聯(lián)苯胺比色法 ;砷含量的測(cè)定方法采用二乙氨基二硫代甲酸銀比色法。結(jié)果 :飼養(yǎng)在牛糞餌料中的蚯蚓纖溶酶活性比飼養(yǎng)于生活垃圾中的蚯蚓纖溶酶活性更高 ,蚯蚓體內(nèi)砷的含量與纖溶酶活性呈正相關(guān) ,而蚯蚓體內(nèi)硒的含量對(duì)纖溶酶活性影響不大。在采用 3種不同的蚯蚓纖溶酶的抽提方法中 ,75 %酒精抽提效率最高 ,0.9%氯化鈉次之 ,10%蔗糖最低 ;在蚯蚓纖溶酶純化過程中 ,透析比超濾對(duì)蚯蚓纖溶酶的活性影響更小一些。結(jié)論 :通過將蚯蚓飼養(yǎng)在適宜的餌料中 ,使用合適的提取手段和提純方法 ,可以提高蚯蚓纖溶酶的提取活性。
中文關(guān)鍵詞:蚯蚓纖溶酶活性 餌料 重金屬 抽提方法
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Factors Influencing the Activity of Fibrinolytic Enzymes From Earthworm, Eisenia foetida
LIU Xiang hui;GE Feng
Abstract:Objective:To study the factors influencing the activity of fibrinolytic enzymes from earthworm and to obtain the better way to extract fibrinolytic enzymes as well as keep its optimum activity. Method:75% alcohol, 0.9%NaCl and 10% saccharose was used to extract the crude fibrinolytic enzymes from earthworm, the method of urokinase gelose fibrin plate was used to measure the activity of fibrinolytic enzymes from earthworm. and the method of 3,3′-diaminobezidine tetrahydrochloride colorimetry to was used measure the content of selenium. The method use ts of measuring the content of arsenic was silver diethyldithiocarbamate colorimetry. Result:The fibrinolytin of earthworms reared with cattle soils had higher activity than that reared with garbage. The arsenic in the earthworm′s body could improve the activity of earthworm′s fibrinolytin. However, the selenium had litter influence on it. Among the three methods of extraction, the 75% alcohol one was the most efficient , the 0.9% NaCl was next, and the 10% saccharose was the lowest. The influence of dialysis on the activity of fibrinolytin was less than that of ultrafiltration, when the earthworm′s fibrinolytin enzyme was further sublimated. Conclusion:The activity of the earthworm′s fibrinolytin will be increased earthworm is reared with the fitting baits and when appropriate methods, of extraction and purification are used.
keywords:activity of the earthworm′s fibrinolytin bait heavy metal method of extraction
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引證文獻(xiàn)(本文共被引2次):
[1] 武金霞,趙曉瑜.蚯蚓纖溶酶的研究進(jìn)展及趨勢(shì)[J].中國(guó)生化藥物雜志,2004,25(5).
[2] 解佰純,李景鵬.蚯蚓纖溶酶的研究進(jìn)展[J].東北農(nóng)業(yè)大學(xué)學(xué)報(bào),2006,37(6):834-837.
