| 公開(kāi)(公告)號(hào) | CN1214113C |
| 公開(kāi)(公告)日 | 2005.08.10 |
| 申請(qǐng)(專利)號(hào) | CN02110673.8 |
| 申請(qǐng)日期 | 2002.01.28 |
| 專利名稱 | 日本血吸蟲中國(guó)大陸株抱雌溝蛋白的基因序列 |
| 主分類號(hào) | C12N15/12 |
| 分類號(hào) | C12N15/12;C12N15/70;C07H21/00;C07K14/44 |
| 分案原申請(qǐng)?zhí)? | |
| 優(yōu)先權(quán) | |
| 申請(qǐng)(專利權(quán))人 | 中國(guó)農(nóng)業(yè)科學(xué)院上海家畜寄生蟲病研究所;上海動(dòng)物生物技術(shù)研究中心 |
| 發(fā)明(設(shè)計(jì))人 | 林矯矯;金亞美;朱建國(guó);馮新港;吳詳甫;;周元聰;蔡幼民 |
| 地址 | 200232 上海市石龍路345弄3號(hào) |
| 頒證日 | |
| 國(guó)際申請(qǐng) | |
| 進(jìn)入國(guó)家日期 | |
| 專利代理機(jī)構(gòu) | 上海新天專利代理有限公司 |
| 代理人 | 衷誠(chéng)宣 |
| 國(guó)省代碼 | 上海;31 |
| 主權(quán)項(xiàng) | 一種編碼為日本血吸蟲抱雌溝蛋白的核苷酸序列，其特征在于該核苷酸序列及編碼相應(yīng)的氨基酸序列如下：gttacactgttatactgaagtagtttaaatgaaatatgtaataataaatcaataagtaat60tatacataacatttaattagtattactgtttttggacaatctataaaatttggtaaaata120tctgacaaagttagttatgccaatccagatcaagcagggtatcatcattgtgcattttgg180cgtttatctgatggtcaatcaattattgcttcatgcactccattcacattacaacgctgt240gggaaaaagttagaattcgattacaaatgttgtgcgggatatgaaaagattggaaaatta300tcatatacatttcgtgatttcaagaaggatgaatgcgctctacaagtatccccatatgaa360ttgtgcatggaatcaattcggaatcactccgatgtatatccattcgca408MetGluSerIleArgAsnHisSerAspValTyrProPheAla1510gaaaaaatgaaaacactcaatgaactcaacacaaagaatccagataaa456GluLysMetLysThrLeuAsnGluLeuAsnThrLysAsnProAspLys15202530ccatacacaatttttgcaccaagtactagaaacgccgagaaatataaa504ProTyrThrIlePheAlaProSerThrArgAsnAlaGluLysTyrLys354045gattttacaaattcagcagaacatattgttccaggtcgattttattta552AspPheThrAsnSerAlaGluHisIleValProGlyArgPheTyrLeu505560aaagatttaaaaagtggactacaattgaatacattagatcctaaacga600LysAspLeuLysSerGlyLeuGlnLeuAsnThrLeuAspProLysArg657075aaactttatgtaacacgagaacttcatggaactgttttagttgattgt648LysLeuTyrValThrArgGluLeuHisGlyThrValLeuValAspCys808590gttgaattagtggatgctgacatggaatgtaactcgggcatcatacat696ValGluLeuValAspAlaAspMetGluCysAsnSerGlyIleIleHis95100105110acaattagatatcctttaacagatacacaaaaactagatagatcatct744ThrIleArgTyrProLeuThrAspThrGlnLysLeuAspArgSerSer115120125attctcagcttactggaatcgaattcagaaactcaatcatttgcaaaa792IleLeuSerLeuLeuGluSerAsnSerGluThrGlnSerPheAlaLys130135140gatttatcagcagcattgaaaaataatttgaaaaatattaactctggt840AspLeuSerAlaAlsLeuLysAsnAsnLeuLysAsnIleAsnSerGly145150155catagatacactgtgattgtaccaagtcaaaaatcctgggaattatta888HisArgTyrThrValIleValProSerGlnLysSerTrpGluLeuLeu160165170cgacaaaagtattcaagtgaacaactacaaagaattgcagctaatcat936ArgGlnLysTyrSerSerGluGlnLeuGlnArgIleAlaAlaAsnHis175180185190gttatcttgaatcagcattgttcaggacatttaattcaatcacttgaa984ValIleLeuAsnGlnHisCysSerGlyHisLeuIleGlnSerLeuGlu195200205aattataaatcattattaggagaaaaacttgaattcatttgtgaaact1032AsnTyrLysSerLeuLeuGlyGluLysLeuGluPheIleCysGluThr210215220gattcaactggtaaagaaacgacattatgtcgtactatgttcaatgat1080AspSerThrGlyLysGluArgHisTyrValArgThrMetPheAsnAsp225230235aaacatgaattaacaataacagatttaacgtcttcaaatggagtagca1128LysHisGluLeuThrIleThrAspLeuThrSerSerAsnGlyValAla240245250catattattgaagaaccattaattccattttcagttatgacatttaga1176HisIleIleGluGluProLeuIleProPheSerValMetThrPheArg255260265270gaattattacagaacaagcaatatgctgaaaaattgaagtctactgaa1224GluLeuLeuGlnAsnLysGlnTyrAlaGluLysLeuLysSerThrGlu275280285tttctaaaacttcttgctgaatgtgatttgaaagtggaacctggagaa1272PheLeuLysLeuLeuAlaGluCysAspLeuLysValGluProGlyGlu290295300aaatatgctatcgttttaccacaagacagttcaatgaagtggtggtca1320LysTyrAlaIleValLeuProGlnAspSerSerMetLysTrpTrpSer305310315acatattcaccatttcaagatgaatataaacgttttcaagcggataaa1368ThrTyrSerProPheGlnAspGluTyrLysArgPheGlnAlaAspLys320325330gaatatcgttgtcgagtagcacgttaccatgttgtgaaaagtgataat1416GluSerArgCysArgValAlaArgTyrHisValValLysSerAspAsn335340345350cgattgaataatattggcaattttgctagtcatacactaggtcatcgt1464ArgLeuAsnAsnIleGlyAsnPheAlaSerHisThrLeuGlyHisArg355360365acaattaatccaaacgaagctctgtatgaaacaacttattttaagaag1512ThrIleAsnProAsnGluAlaLeuTyrGluThrThrTyrPheLysLys370375380actccatatggatcacagctgaatttccattattctccagtcaataat1560ThrProTyrGlySerGlnLeuAsnPheHisTyrSerProValAsnAsn385390395cttgaatcatatgaattagatgatgtgacaatctacgttactccaagg1608LeuGluSerTyrGluLeuAspAspValThrIleTyrValThrProArg400405410atcaatgttccacctgaagtaaatatgactgacataataaacaatcga1656IleAsnValProProGluValAsnMetThrAspIleIleAsnAsnArg415420425430gctgatacaacaatagcgaaaaatcaaacagaaagagctaaaatggat1704AlaAspThrThrIleAlaLysAsnGlnThrGluArgAlaLysMetAsp435440445gaaaaatattttagaaagaatgcacctaaaaatttatttcttgttaca1752GluLysTyrPheArgLysAsnAlaProLysAsnLeuPheLeuValThr450455460acagataatggttggaaggatccaagagctacacagacaacaattaat1800ThrAspAsnGlyTrpLysAspProArgAlaThrGlnThrThrIleAsn465470475ccatataaacctgaattaaatatgtatactgataagaatttagaaagt1848ProTyrLysProGluLeuAsnMetTyrThrAspLysAsnLeuGluAsn480485490tacttactgcttaaccatataccattgtatttatggggtggagatatt1896TyrLeuLeuLeuAsnHisIleProLeuTyrLeuTrpGlyGlyAspIle495500505510ggttatttcgaaaaaaatacagtacataaatttatgtcttctgctggc1944GlyTyrPhe |
| 摘要 | 本發(fā)明涉及生物基因領(lǐng)域，具體涉及日本血吸蟲抱雌溝蛋白的基因序列及在大腸桿菌中的表達(dá)。本發(fā)明根據(jù)已報(bào)道的曼氏血吸蟲編碼抱雌溝蛋白的基因SmGcp序列和編碼日本血吸蟲抱雌溝蛋白保守區(qū)的cDNA序列SjGcp1設(shè)計(jì)兩對(duì)引物，分別擴(kuò)增出日本血吸蟲抱雌溝蛋白編碼序列的5’和3’區(qū)段，再根據(jù)測(cè)序結(jié)果設(shè)計(jì)編碼全長(zhǎng)引物，從蟲體中抽提總RNA，用PCR克隆出該基因，克隆后經(jīng)全序列測(cè)定表明該成熟蛋白cDNA編碼1932個(gè)核苷酸，即643個(gè)氨基酸。該基因的成功克隆及基因序列測(cè)定為以后闡明血吸蟲的生殖發(fā)育機(jī)理，以及從阻滯雌蟲成熟方面防治血吸蟲病打下了良好的基礎(chǔ)。 |
| 國(guó)際公布 |
