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醫(yī)學(xué)免費(fèi)論文:祛風(fēng)通絡(luò)方對阿霉素腎病大鼠腎小球基底膜陰離子位點(diǎn)的影響
【摘要】 目的 觀察祛風(fēng)通絡(luò)方對阿霉素腎病大鼠腎小球基底膜(GBM)上陰離子位點(diǎn)(AS)的影響。方法 尾靜脈注射阿霉素建立阿霉素腎病(AN)大鼠模型，并隨機(jī)分為A(空白組)、B(模型組)、C(祛風(fēng)通絡(luò)方組)、D(激素+祛風(fēng)通絡(luò)方組)、E(激素組)、F(苯那普利組)組，并給予相應(yīng)干預(yù);第3、7周留取腎皮質(zhì)，以醋酸鈾和檸檬酸鉛雙重染色法，透射電鏡觀察GBM的超微結(jié)構(gòu);以PEI示蹤染色電鏡觀察GBM上AS的分布變化。結(jié)果 ①3周時(shí)與A組相比，模型組大鼠24h尿蛋白顯著升高(P<0.01)，血清ALB明顯下降(P<0.01)，血清TG和TCH明顯升高(P<0.01);②3周時(shí)透射電鏡示模型組大鼠腎小球足突呈部分或彌漫性融合，GBM上AS明顯減少;③7周時(shí)與B組相比，C、E組超微結(jié)構(gòu)明顯改善，AS分布呈連續(xù)點(diǎn)線狀分布，平均AS表達(dá)量增強(qiáng)(P<0.01)。結(jié)論 祛風(fēng)通絡(luò)方可通過改善GBM上AS的表達(dá)與分布而修復(fù)腎小球電荷屏障。
【關(guān)鍵詞】 阿霉素腎病;蛋白尿;陰離子位點(diǎn);祛風(fēng)通絡(luò)方醫(yī).學(xué)全.在.線網(wǎng)站www.gydjdsj.org.cn
Effects of Qufengtongluo Recipe on anionic site in glomerular basement membrane in adriamycin-induced nephropathy ratsREN Yan-yun, SUN Wan-sen, ZHAO Yan-long, MA Qiao-ya, WANG ZhuDepartment of Traditional and Western Medicine, the Second Affiliated Hospital,dical School of Xian Jiaotong University, Xian 710004, ChinaABSTRACT: Objective To investigate the expression of anionic site in adriamycin-induced nephropathy (AN) rats, and to further explore the effects of Qufengtongluo Recipe on charge barrier in glomerulus in AN rats. Methods Adriamycin nephropathy was induced by a single tail intravenous injection of adriamycin. Totally 80 rats were randomly divided into normal control group and nephropathy model group. Three weeks later, the nephropathy model was established, and 50 AN rats were randomly divided into five groups: nephropathy model group (B, n=10), Qufeng group (C, n=10), prednisone and Qufeng group (D, n=10), prednisone group (E, n=10) and benazepri group (F, n=10), and they were treated respectively. With treatment being given respectively, renal tissue samples in each group were collected at week 3 and 7, respectively. The ultrastructure and expression of anionic site were examined by electron microscope observation. Results ① After adriamycin injection, a significant increase of the 24-hour urinary protein was observed at week 3 (P<0.01). In AN rats, serum albumin was decreased (P<0.01) while serum TCH and TG were increased (P<0.01). ② In AN rats the diffuse fusion and effacement of foot processes and decrease of anionic sites in GBM were observed at week 3. ③ At week 7, the average intensity of AS dramatically increased in C and E groups (P<0.01) compared with that in nephropathy model group. Conclusion The abnormal expression of AS is the important mechanism that leads to the occurrence and development of proteinuria in AN rats. It is possible that Qufengtongluo Recipe has effects on nephrotic syndrome through altering the charge barrier in GBM in glomerulus.
KEY WORDS: adriamycin nephropathy; proteinuria; anionic site; Qufengtongluo Recipe
腎性蛋白尿的發(fā)生由腎小球?yàn)V過膜的機(jī)械屏障和電荷屏障損傷所致，腎小球毛細(xì)血管壁的電荷選擇性通透性的變化是其發(fā)生的主要原因。腎小球基底膜(glomerular basement membrane, GBM)通過表面豐富的陰離子電荷和網(wǎng)絡(luò)樣小孔徑篩網(wǎng)結(jié)構(gòu)發(fā)揮電荷屏障和孔徑屏障的功能。GBM上陰離子位點(diǎn)(anionic sites, AS)的變化可以有效反應(yīng)腎小球?yàn)V過膜電荷屏障的功能狀態(tài)。祛風(fēng)通絡(luò)方是在“風(fēng)伏腎絡(luò)”[1]的病機(jī)理論指導(dǎo)下組成的中藥復(fù)方，用于腎病臨床多年，效果可靠。本研究通過PEI示蹤法觀察祛風(fēng)通絡(luò)方對阿霉素腎病(adriamycin nephropathy, AN)大鼠GBM超微結(jié)構(gòu)和AS的表達(dá)的影響，為祛風(fēng)通絡(luò)方的臨床應(yīng)用提供新的理論依據(jù)。
