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醫(yī)學(xué)免費(fèi)論文:血必凈對大鼠急性胰腺炎保護(hù)作用機(jī)制探討
【摘要】 目的 探討血必凈對大鼠急性胰腺炎的保護(hù)作用及機(jī)制。方法 SD大鼠48只, 隨機(jī)分為假手術(shù)組、胰腺炎組和治療組，每組再分為4 h、8 h亞組。假手術(shù)組僅行開關(guān)腹手術(shù)，胰腺炎組以牛磺膽酸鈉造模，治療組以血必凈干預(yù)。光鏡觀察胰腺組織病理改變，免疫組化法測胰腺組織腫瘤壞死因子(TNF)-α、干擾素(IL)-1β。結(jié)果 治療組胰腺組織損傷較胰腺炎組明顯減輕，治療組胰腺組織中TNF-α、IL-1β含量顯著低于胰腺炎組 (P<0.01)。結(jié)論 血必凈可能通過抑制TNF-α、IL-1β的產(chǎn)生減輕胰腺炎癥。
【關(guān)鍵詞】 血必凈;胰腺炎;腫瘤壞死因子;干擾素
Research on the mechanism of the protective effects of Xuebijing on acutepancreatitis in rats
ZHANG Yunmin醫(yī).學(xué).全.在.線gydjdsj.org.cn
(Department of General Surgery,The 97th Hospital of PLA, Xuzhou, Jiangsu 221004, China)
Abstract： Objective To investigate the protective effect of Xuebijing (XBJ) on acute pancreatitis in rats and its possible mechanism. Methods 48 SD rats were randomized into 3 groups: sham group, pancreatitis group and XBJ group, each group sub-divided into 4h and 8h subgroups. In the sham groups, the rats only underwent opening and closure of the abdomen; the pancreatitis groups received taurocholate sodium administration to establish animal model and the treatment groups were intervened by Xuebijing. The pathologic changes of pancreatic tissues were observed by optic microscopy. The expressions of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in pancreatic tissues were determined by immunohistochemical methods. Results The histomorphological changes of the pancreatic tissues in XBJ group were much more relieved than those in the pancreatitis group. The expressions of TNF-α and IL-1β were significantly lower in XBJ group than those in the pancreatitis group (P<0.01). Conclusion XBJ could relieve acute pancreatitis in rats by inhibiting the production of TNF-α and IL-1β.
Key words: Xuebijing; pancreatitis; tumor necrosis factor-α; interleukin-1β
