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醫(yī)學(xué)免費論文:布洛芬聚乙二醇b聚L乳酸電紡超細(xì)纖維氈的藥物釋放
【摘要】 目的 通過靜電紡絲制備同時包載有布洛芬的聚乙二醇b聚L乳酸纖維氈,并在纖維中添加月桂酸,考察月桂酸是否對纖維中藥物的釋放行為有影響。方法 以聚乙二醇嵌段聚乳酸為載體材料,有機溶液高壓靜電法紡絲法制得同時包載布洛芬和月桂酸的纖維氈,采用ESEM、WAXD和DSC對其進行表征研究,采用HPLC法測定布洛芬在含有蛋白酶K的磷酸緩沖鹽溶液中的釋放行為。結(jié)果 ESEM結(jié)果顯示得到添加有月桂酸的聚乙二醇聚乳酸纖維藥物釋放體系,WAXD掃描顯示纖維表面無藥物結(jié)晶析出,說明其對布洛芬和月桂酸完全包封,藥物體外釋放表明添加月桂酸后,藥物釋放速率加快明顯。結(jié)論 在聚乙二醇聚乳酸超細(xì)纖維添加月桂酸可加快纖維中布洛芬的釋放，蛋白酶K的降解也可加快布洛芬的釋放。
【關(guān)鍵詞】 超細(xì)纖維; 布洛芬; 靜電紡絲; 聚乳酸; 藥物釋放; 月桂酸醫(yī).學(xué)全.在.線網(wǎng)站www.gydjdsj.org.cn
Drug release from PEGPLLA electrospun fibrous mat with ibuprofen entrapped
WANG Hao1,2,CUI Yong3,JIAO Zixue2,CHEN Xuesi2,DENG Yingjie1
(1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun Jinlin 130022,China;3.School of Life Science and Pharmacology,Shenyang Pharmaceutical University,Shenyang 110016,China)
【Abstract】 Objective To prepare poly ethylene glycol blocked poly lactic acid ultrafine fibers with ibuprofen entrapped by electrospinning and add lauric acid into fibers to investigate whether or not lauric acid affects the in vitro release of ibuprofen.Methods Fiber mats were prepared with both ibuprofen and lauric acid added by organic solvent highvoltage electrospinning technique by using poly ethylene glycol blocked poly lactic acid as drug loading material.The characterization of fiber mats was researched by ESEM,WAXD and DSC.The release behavior of ibuprofen in PBS with proteinase K added was investigated by HPLC.Results PEGPLLA fiber drug delivery system with lauric acid added was obtained,WAXD scanning demonstrated that no drug crystal was decomposed and all compounds were perfectly entrapped.After lauric acid addition,ibuprofen released faster.Conclusion Addition of lauric acid could facilitate ibuprofen release from PEGPLLA fibers.Moreover,proteinase K could facilitate ibuprofen release from PEGPLLA fibers.
【Key words】 ultrafine fiber;ibuprofen;electrospinning;poly lactic acid;drug release;lauric acid
靜電紡絲技術(shù)是一種制備微米及納米尺度纖維的新方法,近些年有不少文獻采用此方法將藥物包載在醫(yī)用高分子材料中,進行了一些前瞻性的研究[15]。載藥纖維制備的大體過程包括將藥物溶于或分散于待紡絲的高分子聚合物有機溶液中或熔體中,將聚合物溶液或熔體帶上103～104 V高壓靜電,在強靜電場作用下帶電的聚合物溶液滴在電場的作用力下克服液體表面張力于毛細(xì)管的末端形成Taylor錐體,并以靜電力為牽引力來形成噴射流進行紡絲,在此過程中,由于溶劑的快速揮發(fā),藥物將以極小的顆粒或分子(離子)狀態(tài)存在于聚合物纖維中最終在接收裝置上獲取一種新型的藥物釋放系統(tǒng)——超細(xì)纖維[6，7],紡絲裝置及原理如圖1所示。
能夠進行靜電紡絲的高分子材料有很多,聚L乳酸是脂肪族聚酯中在人體中生物相容性和生物可降解性最好的材料之一,并且由于其親脂的化學(xué)結(jié)構(gòu),其可對許多在水中溶解度低的化合物進行包裹,是近些年研究應(yīng)用較多的一類高分子藥物載體材料[8，9]。將PLLA用適當(dāng)分子量的聚乙二醇(PEG)修飾后可使高分子呈現(xiàn)一定的親水性,改善其在體內(nèi)的應(yīng)用[10]。本實驗擬通過對溶解在有機溶劑中的聚乳酸進行靜電紡絲制備得到超細(xì)生物可降解纖維。
