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醫(yī)學免費論文:基質金屬蛋白酶-3、10在子宮內膜腺癌組織中的表達
【摘要】 目的 了解基質金屬蛋白酶-3、10(MMP-3、MMP-10)在子宮內膜腺癌組織中的表達情況，及與病變的浸潤程度、組織學分級、臨床分期等方面的關系，探討MMP-3、MMP-10在子宮內膜癌的發(fā)病、浸潤和轉移中的作用及意義。方法 采用免疫組織化學方法分別檢測42例子宮內膜癌、12例非典型增生、12例正常子宮內膜組織中MMP-3、MMP-10的表達，并進行統(tǒng)計學分析。結果 本實驗結果顯示，子宮內膜腺癌、子宮內膜不典型增生過長和正常子宮內膜組織中MMP-3陽性表達率分別為73.8%(31/42)、25.0%(3/12)、8.3%(1/12)，MMP-10陽性表達率分別為76.2%(32/42)、33.3%(4/12)、0%(0/12)。MMP-3、MMP-10的表達均呈逐漸下降趨勢，每兩組的統(tǒng)計學檢驗顯示，MMP-3、MMP-10在子宮內膜腺癌與子宮內膜不典型增生過長、子宮內膜腺癌與正常子宮內膜組織之間，均有統(tǒng)計學差異(P<0.01);;MMP-3、MMP-10的表達與組織學分級、肌層浸潤程度、臨床分期均有關(P<0.01);MMP-3、MMP-10在子宮內膜腺癌中的表達有明顯相關性(P<0.01)，聯(lián)合檢測MMP-3、MMP-10可提高子宮內膜腺癌的檢出率。結論 MMP-3與MMP-10可作為子宮內膜腺癌的腫瘤標志物之一，且聯(lián)合檢測MMP-3與MMP-10可提高子宮內膜腺癌的檢出率。
【關鍵詞】 子宮內膜腺癌;基質金屬蛋白酶-3;基質金屬蛋白酶-10醫(yī).學全.在.線網(wǎng)站www.gydjdsj.org.cn
Expressions of matrix metalloproteinase-3 and matrix metalloproteinase-10 in endometrial carcinomaFENG Jun, GOU Wen-li, LIU Dan, LI XuDepartment of Peripheral Angiography; Department of Gynecology & Obstetrics;Center of Molecule Experiment, the FirstAffiliated Hospital Medical School of Xian Jiaotong University, Xian 710061, ChinaABSTRACT: Objective To explore the expression of matrix metalloproteinase-3 (MMP-3) and matrix metalloproteinase-10 (MMP-10) in normal endometrium, atypical hyperplasia and endometrial adenocarcinoma. We aimed to study the relationship of MMP-3 and MMP-10 expressions with infiltration depth, histologic grade and clinical phase in order to explore the role of MMP-3 and MMP-10 in the occurrence, infiltration and metastasis of endometrial adenocarcinoma. Methods The expressions of MMP-3 and MMP-10 were measured by immunohistochemistry in 12 cases of normal endometrium, 12 cases of atypical hyperplasia and 42 cases of endometrial adenocarcinoma. Results The expression of MMP-3 was 73.8% (31/42), 25.0% (3/12) and 8.3% (1/12) in endometrial adenocarcinoma, atypical hyperplasia and normal endometrium, respectively, whereas the expression of MMP-10 was 76.2% (32/42), 33.3% (4/12) and 0(0/12), respectively. Both MMP-3 and MMP-10 expressions exhibited a pattern of decreased intensity in endometrial carcinoma, atypical hyperplasia and normal endometrium. Furthermore, the statistical analysis showed that the expression of both proteins was significantly greater in endometrial carcinoma than in atypical hyperplasia and normal tissue (P<0.01). Expressions of MMP-3 and MMP-10 had a significant correlation with such clinical parameters as histologic grade, depth of myometrial infiltration and clinical stage (P<0.01). Conclusion MMP-3 and MMP-10 can be used as tumor markers of endometrial carcinoma, and the combined detection of them can increase the detection rate of endometrial carcinoma.
KEY WORDS: endometrial carcinoma; matrix metalloproteinase-3; matrix metalloproteinase-10
惡性腫瘤侵襲和轉移的先決條件是細胞外基質(extracellular matrix, ECM)的降解。ECM是由膠原、蛋白多糖及糖蛋白等成分構成的細胞外骨架結構。基質的降解和基底膜的重塑及穿出血管壁進入微環(huán)境的過程，對于癌細胞侵襲轉移過程來說是關鍵環(huán)節(jié)。研究表明，癌細胞可產(chǎn)生大量分解酶降解基質或基底膜。這些分解酶與癌侵襲轉移能力顯著相關。基質金屬蛋白酶(matrix metalloproteinase, MMPs)是一組調節(jié)復雜的，鋅離子依賴性的內肽酶，是一多基因家族，是人體內降解ECM的主要酶類，是一類與腫瘤浸潤和轉移密切相關的生物酶。MMPs 可以通過降解ECM、調節(jié)細胞凋亡、增加細胞黏附能力、促進新血管生成來促進腫瘤細胞的生長、侵襲和轉移，從而參與腫瘤演進等眾多生理和病理過程[1]�；|溶解素是MMPs中非常重要的一個種類，包括MMP-3、MMP-7、MMP-10、MMP-11等，可以降解ECM的主要結構，包括Ⅳ、Ⅴ、Ⅸ型膠原、纖維連接蛋白、層粘蛋白、蛋白多糖等。目前，對基質溶解素在腫瘤侵襲與轉移中的作用研究較少，尤其是基質溶解素-1(MMP-3)、基質溶解素-2(MMP-10)在子宮內膜癌中的作用國內外報道罕見。因此，探討子宮內膜癌組織中MMP-3、MMP-10的表達及其相關性具有一定的創(chuàng)新性和實用性。本研究應用免疫組化方法檢測正常子宮內膜、不典型增生子宮內膜和子宮內膜腺癌組織中MMP-3和MMP-10的蛋白表達情況，以及其表達變化與組織學分級、肌層浸潤程度、臨床分期的關系，并結合臨床病理特征，初步探討MMP-3和MMP-10與子宮內膜腺癌發(fā)生、發(fā)展的關系及其臨床意義。
