大黃素對模擬冷缺血再灌注后肝細胞內鈣離子濃度及細胞凋亡的影響

醫(yī)學論文范文:大黃素對模擬冷缺血再灌注后肝細胞內鈣離子濃度及細胞凋亡的影響

【摘要】 目的 研究中藥大黃素對模擬冷缺血再灌注后肝細胞內鈣離子濃度及細胞凋亡的影響。方法 體外培養(yǎng)肝細胞株HL��7702，隨機分為對照組和大黃素處理組。對照組未予大黃素處理，大黃素處理組按100、10、1μmol/L分為高、中、低3個濃度組，建立模擬冷缺血再灌注模型，流式細胞技術檢測各組細胞內鈣離子濃度及細胞凋亡水平，分別檢測各組細胞培養(yǎng)上清液乳酸脫氫酶水平。結果 冷缺血8h再灌注6h后，高、中、低濃度大黃素處理組鈣離子熒光強度分別為(24.12±0.51)、(26.35±1.34)、(39.12±1.94)，均顯著低于對照組的105.29±1.01(P<0.01)。高、中、低濃度大黃素處理組細胞凋亡率分別為(5.46±0.41)%、(10.64±0.64)%、(11.90±0.50)%，均顯著低于對照組的(25.40±1.41)%(P<0.01)。高、中濃度大黃素處理組上清液LDH含量分別為(179.67±18.57)u/L、(198.83±14.22)u/L，顯著低于對照組的(351.33±34.16)u/L(P<0.01)。結論 大黃素可降低模擬冷缺血再灌注后的肝細胞內鈣離子濃度，抑制細胞凋亡，減輕肝細胞損傷。

【關鍵詞】 大黃素;肝細胞;冷缺血再灌注;細胞內鈣離子濃度;凋亡

Effect of Emodin on intracellular calcium concentration ([Ca2+]i) andapoptosis of hepatic cells after simulated cold ischemia�瞨eperfusion

QI Xiang, L�a Yi, SHEN Nai�瞴ing, LIU Chang, LIU Xue�瞞in, WANG Bo

Department of Hepatobiliary Surgery, the First Affiliated Hospital, Medical School ofXi�餫n Jiaotong University, Xi�餫n 710061, China

ABSTRACT: Objective To investigate the effect of Emodin on intracellular calcium concentration ([Ca2+]i) and apoptosis of hepatic cells after simulated cold ischemia�瞨eperfusion. Methods Glucose�瞣xygen deprivation, low temperature, subsequent reoxygenation and rewarming were used to induce ischemia�瞨eperfusion injury model in cultured hepatic cells which were divided into 4 groups: control group and Emodin�瞭reated group(100, 10 and 1μmol/L groups). The intracellular calcium concentration ([Ca2+]i) and apoptosis rate were determined by flow cytometry (FCM) respectively; the content of lactate dehydrogenase (LDH) in supernatant was tested. Results Intracellular calcium fluorescence intensity in Emodin�瞭reated groups of high, medium and low density was 24.12±0.51, 26.35±1.34 and 39.12±1.94, respectively, which were significantly lower than 105.29±1.01 in control group(P<0.01). Apoptosis rate in Emodin�瞭reated groups of high, medium and low density was 0.0546±0.0041, 0.1064±0.0064 and 0.1190±0.0050, respectively, which were significantly lower than 0.2540±0.0141 in control group(P<0.01). LDH was (198.83±14.22)u/L and (179.67±18.57)u/L in Emodin�瞭reated groups of medium and high density respectively, which were significantly lower than (351.33±34.16)u/L in control group(P<0.01). Conclusion Emodin could reduce [Ca2+]i and inhibit apoptosis of hepatic cells after simulated cold ischemia�瞨eperfusion, thus protecting hepatic cells effectively醫(yī).學全.在.線網站www.gydjdsj.org.cn.

KEY WORDS: Emodin; hepatic cell; ischemia�瞨eperfusion injury; intracellular free calcium concentration; apoptosis

大黃素[1��2]化學結構為1，3，8�擦u基��6�布谆�蒽醌，具有抗炎、抗腫瘤、保護肝腎等多種生物學作用。有文獻報道[3��4]，大黃素可對細胞內鈣離子濃度及細胞凋亡產生影響。鈣超載及凋亡均為缺血再灌注損傷機制之一。本課題組前期研究發(fā)現(xiàn)[5]，大黃素對肝細胞體外模擬冷缺血再灌注損傷具有保護作用。該保護作用是否與抑制肝細胞內鈣超載及凋亡有關?大黃素對冷缺血再灌注后肝細胞內鈣離子濃度及凋亡的影響未見相關報道。本研究擬通過觀察大黃素對模擬冷缺血再灌注后肝細胞內鈣離子濃度及凋亡的影響，初步探討其對冷缺血再灌注損傷可能的保護機制，為其在臨床肝移植中的應用提供一定的實驗依據。

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