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醫(yī)學(xué)論文范文:間歇性高糖對大鼠腎小管上皮細(xì)胞NRK-52EA轉(zhuǎn)分化的影響
【摘要】 目的:探討間歇性高糖誘導(dǎo)大鼠腎小管上皮細(xì)胞株NRK-52EA轉(zhuǎn)分化干預(yù)作用。方法:NRK-52E細(xì)胞分為空白組、正常葡萄糖組、高糖組、間歇性高糖組，分別作用72 h后，Western blot檢測轉(zhuǎn)化生長因子β1(TGF-β1)、Ⅰ型膠原、金屬蛋白酶-2(MMP-2)、α平滑肌肌動(dòng)蛋白(α-SMA)以及甲狀旁腺素相關(guān)肽(PTHrP)的表達(dá);CM2H2DCFDA試劑盒檢測活性氧(ROS)含量。結(jié)果:高糖組及間歇性高糖組NRK-52E細(xì)胞TGF-β1、Ⅰ型膠原、MMP2、α-SMA以及PTHrP表達(dá)均上調(diào);間歇性高糖組TGF-β1、MMP2以及α-SMA表達(dá)水平較高糖組高(P均<0.05)，其ROS含量也顯著高于高糖組(P<0.01)。結(jié)論:間歇性高糖能通過氧化應(yīng)激誘導(dǎo)腎小管導(dǎo)管上皮細(xì)胞表達(dá)TGF-β1、MMP2，并促進(jìn)NRK-52EA細(xì)胞轉(zhuǎn)分化為α-SMA細(xì)胞。
【關(guān)鍵詞】 間歇性高糖;NRK-52EA 細(xì)胞;轉(zhuǎn)分化
Effects of Fluctuating High Glucose on the Transdifferentiation of Tubule Endothelial Cell Line NRK-52EA in Mouse LIANG Hua-sheng,ZHONG Yu-huaDepartment of Endocrinology,The Ninth Affiliated Hospital,Guangxi Medical University,Beihai,Guangxi 536000,China)Abstract:Objective To investigate the effects of fluctuating high glucose on the transdifferentiation of tubule endothelial cell line NRK-52EA in mouse.Methods NRK-52EA cells were divided into control group,glucose group,high glucose group,fluctuating high glucose group,the expression of transforming growth factorβ1(TGF-β1),typeⅠcollagen,MMP-2,α-SMA and PTHrP were detected with Western blot,and the content of ROS was determined with CM2H2DCFDA kits after treated for 72 h.Results The expression of TGF-β1,typeⅠcollagen,MMP-2,α-SMA and PTHrP were all up-regulation in high glucose group and fluctutating high glucose group;the expression of TGF-β1,MMP-2,α-SMA and the content of ROS in fluctutating high glucose group was significantly higher than that of high glucose group(P<0.05 or P<0.01).Conclusion Fluctuating high glucose could induce the up-regulation of the expression of TGF-β1,MMP-2 in tubule endothelial cells through oxidative stress,and promote the transdifferentiation from NRK-52EAb to α-SMA.
Key words:Fluctuating high glucose;NRK-52EA;Transdifferentiation
糖尿病血糖間歇波動(dòng)目前認(rèn)為對于糖尿病并發(fā)癥的發(fā)生、發(fā)展起著重要的作用，其危害性甚至高于持續(xù)性血糖升高。腎小管上皮細(xì)胞轉(zhuǎn)分化是腎臟纖維化的重要病理基礎(chǔ)，既往研究發(fā)現(xiàn)間歇性高糖能顯著促進(jìn)內(nèi)皮細(xì)胞、心肌細(xì)胞纖維化進(jìn)程[1-2]，高濃度葡萄糖也可誘導(dǎo)腎臟纖維化[3]，但間歇性高糖對腎小管導(dǎo)管上皮細(xì)胞的研究并不多見。本實(shí)驗(yàn)將觀察不同條件葡萄糖狀態(tài)下大鼠腎小管上皮細(xì)胞中調(diào)控腎小管導(dǎo)管上皮細(xì)胞轉(zhuǎn)分化因子的表達(dá)，以探討糖環(huán)境對腎小管上皮細(xì)胞轉(zhuǎn)分化的影響醫(yī).學(xué).全.在.線gydjdsj.org.cn。
