醫(yī)學論文范文:阿加曲班對大鼠肢體缺血再灌注損傷血液流變學指標影響的實驗研究

醫(yī)學論文范文:阿加曲班對大鼠肢體缺血再灌注損傷血液流變學指標影響的實驗研究

【摘要】 目的 探討阿加曲班對大鼠肢體缺血再灌注損傷對血液流變學指標的影響，為缺血再灌注損傷的防治提供新思路。方法 采用大鼠肢體缺血再灌注模型，將14只Wistar大鼠隨機分為2組，對照組和阿加曲班組，每組7只。2組大鼠均于再灌注2 h后，酶聯(lián)免疫吸附(ELISA)法測定血漿P�策x擇素、細胞間粘附分子��1(ICAM��1)濃度;取血檢測血液流變學指標,包括血漿粘度(ηp)、全血低切還原粘度(Lηre)、全血高切還原粘度(Hηre)、紅細胞壓積(HCT)、紅細胞聚集指數(shù)(EAI)、血沉方程K值(ESRK)、紅細胞剛性指數(shù)(TK)、紅細胞變形指數(shù)(EDI)。結果 大鼠肢體缺血再灌注2 h后，阿加曲班組血漿P�策x擇素濃度、血漿ICAM��1濃度顯著低于對照組(P<0.01)。血液流變學指標與對照組比較，阿加曲班組ηp、Hηre、Lηre、EAI和TK明顯降低(P<0.05)，EDI升高(P<0.05)，而對Hct、ESRK無顯著影響(P<0.05)。結論 阿加曲班能夠降低血漿P選擇素和ICAM��1濃度，進而改善大鼠肢體缺血再灌注損傷所造成的血液流變學異常。

【關鍵詞】 缺血再灌注損傷;選擇素;ICAM��1;血液流變學; 阿加曲班

The effects of argatroban on hemorheological parameters in rats with limb ischemia�瞨eperfusion injury DU Liqing^,MI Shijun,WANG Jinghua.^Department of Danger and Severe Medicine,The Fengrun Distriact People’s Hospital,Hebei,Tangshan 064000,China

【Abstract】 Objective To investigate the effects of agatroban on hemorheological parameters,plasma P�瞫electin and plasma ICAM��1 of rats with limb ischemia/reperfusion injury in order to provide a new method for the prevention and therapy of ischemia/reperfusion injury. Methods 14 Wistar rats were randomly divided into control group and agatroban group, with 7 rats in each group.The rat model of limb ischemia/reperfusion injury was made according to the ZHANG Lianyuan’s ways. The plasma P�瞫electin and plasma ICAM��1 levels were detected by enzyme linked immunosorbent assay (ELISA) at 2h after reperfusion. The blood samples were taken and hemorheological indexes were detected, including plasma viscosity, low shear reduction viscosity, high shear reduction viscosity, hematocrit erythrocyte aggregation index, erythrocyte sedimentation rate K and erythrocyte rigidity index.Results 2h after limb ischemia/reperfusionin rats,the plasma P�瞫electin and plasma ICAM��1 levels in argatroban group were significantly lower than those of control group(P<0.05).As compared with those in control group,the ηp、Hηre、Lηre、EAI and Tk in argatroban group were significantly decreased(P<0.05),however,EDI was obviously increased(P<0.05),but no obvious effect on Hct,ESRK was observed.Conclusion Agatroban can reduce plasma P�瞫electin and plasma ICAM��1 levels,as a result,to improve hemorheological abnormality caused by limb ischemia�瞨eperfusion injury in rats.

【Key words】 ischemia�瞨eperfusion injury; hemorheology; argatroban; P�瞫electin; ICAM��1

近年隨著對缺血再灌注損傷基礎研究的深入，對其再灌注損傷的保護研究也越來越多。試圖應用不同的方法，通過不同的途徑發(fā)揮作用。缺血預處理、抗氧化療法、抗白細胞療法等，都有很好的保護作用[1，2]。阿加曲班是化學合成的低分子藥物,直接與凝血酶的催化活性位點結合,滅活凝血酶。近年研究表明阿加曲班還可抑制凝血酶的其他作用，如可以抑制腫瘤轉移、炎性過程及血管成形術后的再狹窄等[3]，對微循環(huán)障礙和血液流變性也有一定影響[4]。但能否對肢體缺血�苍俟嘧�損傷導致的血液流變學異常有良好的改善作用，我們對大鼠肢體缺血再灌注損傷進行了實驗研究，觀察對比阿加曲班干預和單純?nèi)毖�再灌注損傷導致的血液流變學異常有無顯著性影響，為缺血再灌注損傷的臨床防治提供實驗基礎醫(yī).學全.在.線網(wǎng)站gydjdsj.org.cn。

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