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醫(yī)學(xué)免費論文:誘導(dǎo)的視網(wǎng)膜色素上皮細(xì)胞增生作用及其作用機制
【摘要】 目的：觀察(R, R)XY10和(S, S)XY10對視網(wǎng)膜色素上皮細(xì)胞的增生作用，并且進一步的研究其作用機制。但(R, R)XY10和(S, S)XY10對人臍靜脈內(nèi)皮細(xì)胞并無增生的作用。方法：通過人視網(wǎng)膜色素上皮細(xì)胞(ARPE19)和人臍靜脈內(nèi)皮細(xì)胞(HUVECs)研究(R, R) XY10和(S, S)XY10對視網(wǎng)膜色素上皮細(xì)胞的增生作用，并且采用ERK、KT、PI3K、蛋白激酶C(PKC)和一氧化氮合酶(NOS)抑制劑來研究其作用機制。結(jié)果：(R, R)XY10和(S, S)XY10促進了ARPE19細(xì)胞的增生，并具有劑量依賴性，但是對HUVECs細(xì)胞沒有影響。如果同時加入增生抑制劑H7 (5μmol/L)、金絲桃素(20μmol/L)、PD98059(2μmol/L)、LY294002(50μmol/L)、SH5(10μmol/L)和LNAME (100μmol/L)，則給予H7、金絲桃素、PD98059和LY294002各組的增生作用受到了抑制，而給予SH5和LNAME兩組的增生作用沒有影響。結(jié)論：(R, R)XY10和(S, S)XY10能夠誘導(dǎo)ARPE19細(xì)胞增生，其作用可能是通過MAPK和PI3K的途徑來發(fā)揮該作用。因此，(R, R)XY10和(S, S)XY10能通過修復(fù)損傷的RPE細(xì)胞來治療老年性黃斑變性。
【關(guān)鍵詞】 年齡相關(guān)性黃斑變性;(R,R)XY10; (S,S)XY10; ARPE19 cells;人臍靜脈血管內(nèi)皮細(xì)胞;增生
Controlled continuous curvilinear capsulorhexis in short axial length and shallow anterior chamber eyes
GuangYu Yang, YouLi Huang, ZhiFeng Wu 醫(yī).學(xué).全.在.線gydjdsj.org.cn
Department of Ophthalmology, the Second Peoples Hospital,Wuxi 214002,Jiangsu Province,China
Correspondence to:GuangYu Yang.Department of Ophthalmology, the Second Peoples Hospital,Wuxi 214002,Jiangsu Province,China.wuxiygy@163.com
AbstractAIM：To investigate the efficacy of controlled continuous curvilinear capsulorhexis(CCC) technique in short axial length and shallow anterior chamber eyes.METHODS：Sixtyeight patients(68 eyes) with short axial length and shallow anterior chamber were included.The routine CCC technique was used in 32 cases (32 eyes) and controlled CCC technique was used in 36 cases (36 eyes).The success rate and complication were compared between two groups.RESULTS：The success rate of the routine technique group and controlled technique group was 53.13% and 86.11% respectively. Incomplete CCC leading to posterior capsule tears was 9.38% and zero in two groups respectively.CONCLUSION: Controlled CCC technique can increase the success rate and reduce complications in short axial length and shallow anterior chamber eyes.
