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醫(yī)學(xué)免費論文:動脈蒂植入自體游離移植骨再血管化的實驗研究
【摘要】 目的 研究動脈蒂植入非血管化自體骨后移植骨血管再生的情況。方法 將36只大白兔雙側(cè)橈骨部分截斷，剝離骨膜，分別植入雙側(cè)咬肌區(qū)，其中一側(cè)將遠端結(jié)扎的頜外動脈蒂植入游離橈骨骨髓腔作為實驗側(cè)，另一側(cè)單純移植作為對照側(cè)。術(shù)后3 d、1周、2周、3周、4周、6周處死動物取材，通過組織學(xué)檢查、血管墨汁灌注、CD34免疫組化染色檢測移植骨微血管密度（MVD)，觀察移植骨的再血管化情況。結(jié)果 實驗側(cè)術(shù)后3 d，對照側(cè)術(shù)后2周移植骨出現(xiàn)再血管化。術(shù)后3 d、1周、2周、3周、4周，實驗側(cè)移植骨的再血管化程度高于對照側(cè)；術(shù)后4周，實驗側(cè)再血管化程度達到高峰。結(jié)論 動脈蒂植入非血管化自體骨的方法具有促進移植骨血管生成的作用。
【關(guān)鍵詞】 非血管化自體骨 動脈蒂植入 微血管密度
An experimental study on angiogenesis of non-vascularized autogenous bone graft with vascular bundle implantation XIANG Zhao, YAO Yao, WANG Ya, HUA Cheng-ge, ZHANG Fu-gui, TANG Xiu-fa. （Dept. of Head and Neck Oncology, West China College of Stomatology, Sichuan University, Chengdu 610041, China)
[Abstract] Objective To investigate the effect of vascular bundle implantation in autogenous bone graft on angiogenesis. Methods Thirty-six New Zealand white rabbits were evaluated in this study. A portion of bilateral radial bones of a rabbit were removed as free bone grafts, whose periostea were peeled off. In test group, the external maxillary artery bundle was passed through the marrow cavity of the bone. In control group, there was no vascular bundle implantation. Each bone was placed in masseter muscle separately. The rabbits were sacrificed and the specimens were procured at 3 days, 1, 2, 3, 4 and 6 weeks after surgery for histological observation, Chinese ink perfusion and CD34 immunohistochemistry. Microvessel density（MVD) was assessed in order to evaluate angio-genesis of autogenous bone grafts. Results The bone grafts were found revascularization in 3 days after surgery in the test group, whereas at 2 weeks in the control group. In 3 days, 1 week, 2 weeks, 3 weeks and 4 weeks after surgery, the MVD of test group was significantly higher than that of control group. In 4 weeks after surgery, angiogenesis of test group reached to peak. Conclusion Vascular bundle implantation improved angiogenesis in non-vascularized autogenous bone graft in this study醫(yī).學(xué).全.在.線gydjdsj.org.cn.
[Key words] non-vascularized autogenous bone graft； vascular bundle implantation； microvessel density
對于非血管化自體骨移植，促進其血管生成是骨愈合的關(guān)鍵。本研究利用動物實驗觀察動脈蒂植入非血管化自體骨后移植骨血管生成的情況，并與單純骨移植作比較，為該方法的臨床應(yīng)用提供理論依據(jù)。
1 材料和方法
