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醫(yī)學免費論文:姜黃素對哮喘大鼠肺內粒細胞-巨噬細胞集落刺激因子表達及氣道炎癥的影響
【摘要】 目的 通過觀察姜黃素對哮喘大鼠肺泡灌洗液總細胞數及嗜酸性粒細胞(Eos)計數、肺組織粒細胞-巨噬細胞集落刺激因子(GM-CSF)表達的影響,探討姜黃素對哮喘的治療作用。方法 36只雄性SD大鼠隨機分為正常對照組(A組)、哮喘模型組(B組)、姜黃素治療組(C組)各12只,采用卵蛋白(OVA)致敏大鼠哮喘模型,觀察三組動物哮喘發(fā)作癥狀、肺泡灌洗液總細胞數及嗜酸性粒細胞計數情況及原位雜交方法檢測肺組織中GM-CSFmRNA含量變化。結果 B組大鼠哮喘發(fā)作癥狀最明顯,C組大鼠癥狀輕,A組無癥狀。B組肺泡灌洗液總細胞數及嗜酸性粒細胞計數均明顯高于其余兩組; C組明顯少于B組; A組最少。肺組織中GM-CSFmRNA表達A組微弱表達, C組較強, B組表達最強。結論 姜黃素可抑制哮喘大鼠的氣道炎癥,其作用機制可能是通過抑制GM-CSF的表達而導致嗜酸性粒細胞生成減少及存活時間縮短來實現。
【關鍵詞】 姜黃素 哮喘 粒細胞-巨噬細胞集落刺激因子 氣道炎癥
Effects of curcumine on granulocyte macrophage colony stimulating factor expression and airway inflammation in asthmatic rats
WU Dong, et al.
Department of Respiratory Diseases, the Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524001 China
【Abstract】 Objective To observe the effects of curcumine on granulocyte macrophage colony stimulating factor expression and airway inflammation in asthmatic rats. Methods 36 male Sprague-Dawley rats were randomly divided into normal control(A group), model group(B group),curcumine group(C group), 12 rats in each group. Rats were sensitized and challenged continually with ovalbumin(OVA) for the purpose of induction of asthma in rats. To observe asthmatic attack and airway inflammation in asthmatic rats. Granulocyte macrophage colony stimulating factor in rat pulmonary tissues was detected by using in situ hybridization method. Results Asthmatic attack obviously in B group，gently in C group, Absence of symptoms in A group. The total cell amount in bronchoalveolar ravage fluid and eosin Phil were obviously higher in B group than C group and A group. The expression of GM-CSFmRNA in C group was significantly higher than that in A group and it was lower than that in B group.Conclusions The effect of curcumine on airway inflammation in asthmatic rats may be correlated with its inhibition on over expression of GM-CSF in lung tissue.醫(yī).學全.在.線網站www.gydjdsj.org.cn
【Key words】 Curcumine Asthma Granulocyte macrophage colony stimulating factor Airway inflammation
支氣管哮喘是一種特殊的氣道炎癥,主要表現為氣道高反應與變異性較大的可逆性氣流阻塞。有研究表明[1]，粒細胞-巨噬細胞集落刺激因子(GM-CSF)在炎癥過程中起核心作用。姜黃素因其在抗炎、抗纖維化、抗腫瘤、抗氧化等作用而倍受學者們的關注[2]。本研究通過觀察姜黃素對哮喘大鼠氣道炎癥、肺組織粒細胞-巨噬細胞集落刺激因子(GM-CSF)表達的影響,探討姜黃素對哮喘的治療作用。
