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醫(yī)學(xué)論文范文:美托洛爾對(duì)異丙基腎上腺素充血性心力衰竭大鼠模型TNF-α的影響
【摘要】 目的 探討美托洛爾對(duì)充血性心力衰竭(心衰)大鼠血漿腫瘤壞死因子-α(TNF-α)的影響。方法 雄性SD大鼠80只，隨機(jī)分為空白組(n=10)及模型組(n=70)，模型組皮下注射異丙基腎上腺素2次(170 mg· kg-1)，6周后應(yīng)用超聲心動(dòng)圖檢測，以左室射血分?jǐn)?shù)≤45%確定造模成功，將心衰大鼠模型隨機(jī)分為心衰對(duì)照組(n=13)、美托洛爾組(n=14);18周后使用左心導(dǎo)管進(jìn)行血流動(dòng)力學(xué)檢查，利用放免法檢測血漿TNF-α的改變并觀察心肌病理形態(tài)學(xué)特征。結(jié)果 ①與空白組比較，心衰對(duì)照組心功能明顯惡化，心室質(zhì)量指數(shù)、左心室質(zhì)量指數(shù)及血漿TNF-α顯著升高(P<0.01 )，心室重構(gòu)明顯;②與心衰對(duì)照組比較，美托洛爾組心功能明顯好轉(zhuǎn)，心室質(zhì)量指數(shù)、左心室質(zhì)量指數(shù)及血漿TNF-α明顯降低(P<0.05 )，心室重構(gòu)好轉(zhuǎn)。結(jié)論 美托洛爾能改善心功能，逆轉(zhuǎn)心室重構(gòu)，其作用可能與抑制心衰大鼠血漿TNF-α表達(dá)有關(guān)。
【關(guān)鍵詞】 充血性心力衰竭;異丙基腎上腺素;美托洛爾;大鼠;腫瘤壞死因子-α;心室重構(gòu)
Effect of metoprolol on plasma TNF-α in rat model with isoproterenol-inducedcongestive heart failure
CAI Hui, GUO Han, ZHAO Zhiming, CAI Jiayu, GUO Junhao
(Department of Integrated Traditional Chinese and Western Medicine, Nanjing General Hospital of Nanjing
Military Command, PLA, Nanjing, Jiangsu 210002, China)
Abstract: Objective To explore the effect of metoprolol on plasma tumour necrosis factor-α (TNF-α) in rats with congestive heart failure (CHF). Methods 80 male SD rats were randomly allocated to the isoproterenol (ISO)-induced heart failure group (n=70) receiving a subcutaneous injection of ISO (170 mg per kilogram of body weight) twice and the healthy normal group (n=10) receiving a subcutaneous injection of 0.25 ml of normal saline twice. At the 6th week, the left ventricular ejection fractions (LVEF) were measured by echocardiogram, the remaining rats, with a LVEF≤45%, were randomly divided into two groups: heart failure control group (n=13), metoprolol group (n=14，8 mg/kg-1· d-1, i. g., ). At the 18th week, the hemodynamic parameters were detected, including HR, LVEDP, LVSP and ±dp/dtmax; LVWI was measured after LV morphopathological examination. TNF-α in plasma was examined by radioimmunoassay. Results ①Compared with the normal group, the heart function significantly deteriorated; VWI, LVWI and the plasma TNF-α significantly increased (P<0.01) in the heart failure control group. ②Compared with the heart failure control group, the heart function markedly improved, and the VWI, LVWI and the plasma TNF-α decreased significantly (P<0.05 ) in the metoprolol group, with an improved ventricular remodeling. Conclusion Metoprolol can inhibit the plasma TNF-α, improve heart function and reverse ventricular remodeling with CHF. Its effect might be involved in the inhibition of the expression of plasma TNF-α in hear failure rats醫(yī).學(xué).全.在.線www.gydjdsj.org.cn.
Key words: congestive heart failure; isoproterenol; metoprolol; rats; TNF-α; ventricular remodeling
充血性心力衰竭(congestive heart failure, CHF，心衰)是各種心血管疾病最終的共同轉(zhuǎn)歸，嚴(yán)重威脅著人類的健康。目前研究認(rèn)為心衰是由于神經(jīng)內(nèi)分泌細(xì)胞因子系統(tǒng)的激活而介導(dǎo)的心室重塑過程[1]。在心衰的發(fā)生發(fā)展過程中，血漿腫瘤壞死因子-α(TNF-α)具有降低心功能、促進(jìn)心衰發(fā)展的作用[2-3]。本研究以心衰大鼠為實(shí)驗(yàn)?zāi)Ｐ�，觀察美托洛爾對(duì)血漿TNF-α 的影響并探討其意義。
